Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Molecular cloning and expression analyses of porcine MAP1LC3A in the granulosa cells of normal and miniature pig

doi: 10.1186/1477-7827-11-8

Figure Lengend Snippet: Primers used for the full-length cloning and expression of the porcine MAP1LC3A gene

Article Snippet: The membrane was then incubated for 2 h with anti-rabbit MAP1LC3A monoclonal antibody (diluted 1:1000; Abcam, MA), anti-rabbit ATG5 polyclonal antibody (diluted 1:1000; Abfrontier, Seoul, Kor).

Techniques: Clone Assay, Expressing, Sequencing

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Molecular cloning and expression analyses of porcine MAP1LC3A in the granulosa cells of normal and miniature pig

doi: 10.1186/1477-7827-11-8

Figure Lengend Snippet: Effect of rapamycin treatment on the expression of MAP1LC3A and ATG5 protein expression in cultured granulosa cells. A. Immunofluorescence staining of cultured granulosa cells. B. Western blot analysis of the porcine MAP1LC3A protein. C. Real-time PCR of porcine MAP1LC3A mRNA. Data represent the mean ± standard error of 3 individual experiments (p < 0.05).

Article Snippet: The membrane was then incubated for 2 h with anti-rabbit MAP1LC3A monoclonal antibody (diluted 1:1000; Abcam, MA), anti-rabbit ATG5 polyclonal antibody (diluted 1:1000; Abfrontier, Seoul, Kor).

Techniques: Expressing, Cell Culture, Immunofluorescence, Staining, Western Blot, Real-time Polymerase Chain Reaction